Monkeypox Mutations Causing Virus to Spread Fast, Evade Drugs and Vaccines
Published: 2022-11-05 - Updated: 2023-01-04
Author: University of Missouri-Columbia | Contact: missouri.edu
Peer-Reviewed Publication: Yes
Journal Reference: DOI Link to the Study Paper
Library: Monkeypox Virus Publications
Synopsis: Monkeypox has infected over 77,000 people in more than 100 countries worldwide, and mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. We could see how the virus has evolved by doing a temporal analysis. A key finding was that the virus is now accumulating mutations, specifically where drugs and antibodies from vaccines are supposed to bind. Our focus is on looking at the specific genes involved in copying the virus genome, and monkeypox is a massive virus with approximately 200,000 DNA bases in the genome. The DNA genome for monkeypox is converted into nearly 200 proteins, so it comes with all the 'armor' it needs to replicate, divide and continue to infect others. Viruses will make billions of copies of themselves, and only the fittest will survive as the mutations help them adapt and spread.
Monkeypox is a zoonotic pox virus infection that can occur in humans and other animals. Monkeypox in humans and animals is caused by infection with the monkeypox virus - a double-stranded DNA virus in the genus Orthopoxvirus, family Poxviridae. Two recognized distinct types are the Congo Basin clade and the milder West African clade.
Mutations in the monkeypox virus replication complex: Potential contributing factors to the 2022 outbreak
Monkeypox has infected more than 77,000 people in more than 100 countries worldwide, and - similar to COVID-19 - mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines in its mission to infect more people.
Now, a team of researchers at the University of Missouri has identified the specific mutations in the monkeypox virus that contribute to its continued infectiousness. The findings could lead to several outcomes: modified versions of existing drugs used to treat people suffering from monkeypox or the development of new drugs that account for the current mutations to increase their effectiveness at reducing symptoms and the spread of the virus.
Kamlendra Singh, a professor in the MU College of Veterinary Medicine and Christopher S. Bond Life Sciences Center principal investigator, collaborated with Shrikesh Sachdev, Shree Lekha Kandasamy, and Hickman High School student Saathvik Kannan to analyze the DNA sequences of more 200 strains of monkeypox virus spanning multiple decades, from 1965, when the virus first started spreading, to outbreaks in the early 2000s and again in 2022.
"By doing a temporal analysis, we were able to see how the virus has evolved, and a key finding was the virus is now accumulating mutations, specifically where drugs and antibodies from vaccines are supposed to bind," Sachdev said. "So, the virus is getting smarter; it can avoid being targeted by drugs or antibodies from our body's immune response and continue to spread to more people."
Needles in a Haystack
Singh has been studying virology and DNA genome replication for nearly 30 years. He said the homology, or structure, of the monkeypox virus, is very similar to the vaccinia virus, which has been used as a vaccine to treat smallpox. This enabled Singh and his collaborators to create an accurate, 3D computer model of the monkeypox virus proteins and identify where the specific mutations are located and their functions in recently contributing to the virus becoming so infectious.
"Our focus is on looking at the specific genes involved in copying the virus genome, and monkeypox is a huge virus with approximately 200,000 DNA bases in the genome," Singh said. "The DNA genome for monkeypox is converted into nearly 200 proteins, so it comes with all the 'armor' it needs to replicate, divide and continue to infect others. Viruses will make billions of copies of themselves, and only the fittest will survive, as the mutations help them adapt and spread."
Kannan and Kandasamy examined five specific proteins while analyzing the monkeypox virus strains: DNA polymerase, DNA helicase, bridging protein A22R, DNA glycosylase, and G9R.
"When they sent me the data, I saw that the mutations were occurring at critical points impacting DNA genome binding, as well as where drugs and vaccine-induced antibodies are supposed to bind," Singh said. "These factors are surely contributing to the virus' increased infectivity. This work is important because the first step toward solving a problem is identifying where the problem is specifically occurring in the first place, and it is a team effort."
The Evolution of Viruses
Researchers continue questioning how the monkeypox virus has evolved. The efficacy of current CDC-approved drugs to treat monkeypox has been suboptimal, likely because they were originally developed to treat HIV and herpes but have since received emergency use authorization in an attempt to control the recent monkeypox outbreak.
"One hypothesis was when patients were being treated for HIV and herpes with these drugs, they may have also been infected with monkeypox without knowing, and the monkeypox virus got smarter and mutated to evade the drugs," Singh said. "Another hypothesis is that the monkeypox virus may be hijacking proteins in our bodies and using them to become more infectious and pathogenic."
Singh and Kannan have collaborated since the COVID-19 pandemic began in 2020, identifying the specific mutations causing COVID-19 variants, including Delta and Omicron. The United Nations recently recognized Kannan for supporting their 'Sustainable Development Goals,' which help tackle the world's greatest challenges.
"I could not have done this research without my team members, and our efforts have helped scientists and drug developers assist with these virus outbreaks, so it is rewarding to be a part of it," Singh said.
"Mutations in the monkeypox virus replication complex: Potential contributing factors to the 2022 outbreak" was recently published in Journal of Autoimmunity. Co-authors on the study include Shrikesh Sachdev, Athreya Reddy, Shree Lekha Kandasamy, Siddappa Byrareddy, Saathvik Kannan, and Christian Lorson.
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