Hearing Impairment in Novel Preclinical Model of Autism

Author: Medical University of South Carolina
Published: 2022/12/25 - Updated: 2023/01/03
Peer-Reviewed: Yes
Contents: Summary - Main - Related Publications

Synopsis: Researchers uncover hearing impairment in a novel preclinical model of autism in Beyond the brain: A link between hearing loss and autism spectrum disorder (ASD). Many patients with ASD show increased sensitivity to sound. While many scientists in the past have looked to the brain for an underlying cause, the MUSC team took a different approach by studying the peripheral hearing system. The findings from this study could be important not only for patients who are MEF2C deficient but also for people with ASD or hearing loss as a whole.

Syndromic Autism

Autism is a common and heritable neuropsychiatric disorder that can be categorized into syndromic and non-syndromic, the former of which are associated with other neurological disorders or syndromes. Syndromic Autism spectrum disorder (ASD) is a disorder with a clinically defined pattern of somatic abnormalities and a neurobehavioral phenotype that may include ASD. Most have a known genetic cause. Examples include fragile X syndrome and tuberous sclerosis complex. The diagnosis is typically confirmed by targeted genetic testing for trisomy 21 or fragile X syndrome (FXS).

Main Digest

Peripheral Auditory Nerve Impairment in a Mouse Model of Syndromic Autism.

A cross-disciplinary team of researchers in the College of Medicine at the Medical University of South Carolina (MUSC) has discovered hearing impairment in a preclinical model of autism spectrum disorder (ASD). More specifically, the researchers report in the Journal of Neuroscience that they observed mild hearing loss and defects in auditory nerve function. A closer examination of the nerve tissue revealed abnormal supportive cells called glia, aging-like degeneration, and inflammation. The findings from this study highlight the importance of considering sensory organs and their interactions with the brain in understanding ASD.

Many patients with ASD show increased sensitivity to sound. While many scientists in the past have looked to the brain for an underlying cause, the MUSC team took a different approach by studying the peripheral hearing system.

"Hearing impairment may have an impact on the higher-level auditory system and, eventually, cognitive function," said Hainan Lang, M.D., Ph.D., professor in the Department of Pathology and Laboratory Medicine at MUSC and one of two senior authors of the study.

Jeffrey Rumschlag, Ph.D., a postdoctoral researcher in the MUSC Hearing Research Program, is a co-first author of the manuscript.

Previous studies of aging-related hearing loss showed that the brain could increase its response to make up for reduced auditory signals from the inner ear. Lang wanted to determine if this increase, called central gain, could contribute to abnormal brain response to sound in ASD. However, a significant obstacle lay in her path.

"We didn't have a clinically relevant model to test this important fundamental question directly," she said.

The preclinical model that would allow Lang to test her hypothesis was developed in the lab of Christopher Cowan, Ph.D., chair of Neuroscience at MUSC. Mice in this model have only one working copy of a gene called MEF2C. Cowan's group had studied MEF2C in the past for its role in brain development and found that it was important for regulating circuit formation in the brain. They became especially interested in creating a preclinical model when a group of patients with ASD-like symptoms were identified with MEF2C mutations. Cowan's models also show ASD-like behaviors, including increased activity, repetitive behavior, and communication deficits.

Continued below image.
Expression of the MEF2C protein (green) in the nuclei of neuronal cells (stained with a neuronal marker protein in red) in the inner ear of a young adult mouse. Nuclei were stained with Dapi (blue). Image courtesy of Dr. Hainan Lang of the Medical University of South Carolina - Image Credit: Dr. Hainan Lang, Medical University of South Carolina.
Expression of the MEF2C protein (green) in the nuclei of neuronal cells (stained with a neuronal marker protein in red) in the inner ear of a young adult mouse. Nuclei were stained with Dapi (blue). Image courtesy of Dr. Hainan Lang of the Medical University of South Carolina - Image Credit: Dr. Hainan Lang, Medical University of South Carolina.
Continued...

Lang and Cowan's collaboration began as they presented posters side by side at an orientation for the College of Graduate Studies at MUSC. Lang's lab had identified molecular regulators, including MEF2C, crucial for inner ear development. She saw Cowan's model as something she could use to test her hypothesis about hearing loss in neurodevelopmental diseases. Cowan enthusiastically agreed, and the research team began to assess the ability of the MEF2C-deficient mice to hear.

They first measured the brain's response to auditory signals using a modified version of a test commonly used to screen newborn infants for hearing loss. Mild hearing loss was observed in the mice with only one working copy of MEF2C, while hearing remained normal in those with two working copies. To investigate this loss further, the researchers measured the activity of the auditory nerve, which carries signals from the inner ear to the brain. They found reduced activity in this nerve in mice with only one copy of MEF2C.

With their sights set on the auditory nerve, the researchers used advanced microscopes and staining techniques to determine what was going wrong. Although the overall hearing sensitivity loss was mild, the researchers were excited to see a big difference in auditory nerve response. Nerves from mice with a single copy of MEF2C showed cellular degeneration much like that seen in age-related hearing loss. The researchers also saw signs of increased inflammation, with disrupted blood vessels and activated immune cells called glia and macrophages. This finding was especially surprising to the researchers.

"Glial cells were not my first thought; I thought it was a neuronal change," said Lang. "Now we understand that auditory nerve activity can also involve the immune system, and that's the beautiful new direction we want to continue to study."

Cowan also believes that the finding opens the way for a new area of neuroscience research.

"We have more appreciation now that there is an important interaction between the immune system in your body and the immune system in your brain," he said. "The two systems play critical roles in shaping how nervous system cells communicate with each other, in part, by pruning excess or inappropriate connections that have formed. This is an essential aspect of healthy brain development and function."

The findings from this study could be important not only for patients who are MEF2C deficient but also for people with ASD or hearing loss as a whole.

"Understanding how this gene may be participating in ear development and how the inner ear development is affecting brain development has tremendous applicability," said Cowan.

In future studies, the researchers aim to discover how exactly MEF2C causes the changes identified in this study. The research team also hopes to explore these findings in patients with MEF2C deficiency using noninvasive hearing tests.

Lang and Cowan both emphasize the importance of collaboration across disciplines for allowing studies like this to take place.

"The power of collaboration is tremendous for a place like MUSC," said Cowan. "This collaboration, for us, was ideal because Dr. Lang is an expert in hearing function and development, whereas I am more the genetics and molecular development person. These kinds of collaborations are ideal, and it's precisely what MUSC is encouraging a lot of us to think about doing more and more."

"In other words, we each play different instruments so, together, we can make a better harmony," said Lang.

Other MUSC collaborators included Jeremy Barth, Ph.D., of the Department of Regenerative Medicine and Cell Biology; Kelly Harris, Ph.D., and Carolyn McClaskey, Ph.D., of the Department of Otolaryngology and Head and Neck Surgery; and Martin Romeo, Ph.D., and Silvia Vaena, Ph.D., of the MUSC Hollings Cancer Center.

Attribution/Source(s):

This peer reviewed publication pertaining to our Autism Information section was selected for circulation by the editors of Disabled World due to its likely interest to our disability community readers. Though the content may have been edited for style, clarity, or length, the article "Hearing Impairment in Novel Preclinical Model of Autism" was originally written by Medical University of South Carolina, and submitted for publishing on 2022/12/25 (Edit Update: 2023/01/03). Should you require further information or clarification, Medical University of South Carolina can be contacted at web.musc.edu. Disabled World makes no warranties or representations in connection therewith.

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