How Muscles Work Study Show Insight into Muscle Disorders

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Scientists led by Kristina Djinović-Carugo at the Max F. Perutz Laboratories (MFPL) of the University of Vienna and the Medical University of Vienna have elucidated the molecular structure and regulation of the essential muscle protein actinin. The new findings allow unprecedented insights into the protein's mode of action and its role in muscle disorders. The findings, made in collaboration with King's College London (KCL), may lead to improved treatments, and are published in the top-class journal Cell.

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Most animals rely on muscles to move, irrespective whether it is feed, fight or flee. The smallest building block of a muscle is the sarcomere, hundreds of which are successively arranged to form muscle fibers.

"Sarcomeres are mainly made up of actin and myosin protein strands called filaments. Muscle shortening or contraction depends on these filaments sliding against each other, and requires that the actin filaments are anchored in planes, called Z-discs. The major Z-disc protein is actinin, which is also responsible for anchoring another protein called titin. Titin ensures that the actin and myosin filaments of a sarcomere are positioned correctly and re-adjusts the sarcomere to its original length after muscle activity", explains structural biologist Kristina Djinović-Carugo.

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Important Role in Heart and Muscle Disorders

Actinin is a micro-filament protein. Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal muscle cells, and to the dense bodies in smooth muscle cells. The functional protein is an anti-parallel dimer, which cross-links the thin filaments in adjacent sarcomeres, and therefore coordinated contractions between sarcomeres in the horizontal axis. The non-sarcomeric actinins (ACTN1 and ACTN4) are widely expressed. Both ends of the rod-shaped actinin dimer contain actin-binding domains.

Actinin is clearly an essential protein - embryos of organisms that cannot produce it die. If, however, actinin is produced but does not function properly, the organism suffers from diseases such as muscular dystrophies and cardiomyopathies. Knowing the detailed structure of actinin and muscle would not only better our understanding of the role of mutant proteins in disease, but would also aid accurate diagnosis and may ultimately assist designing new therapies.

Muscle protein structure is a research topic of Kristina Djinović-Carugo at the Max F. Perutz Laboratories (MFPL) of the University of Vienna and the Department for Structural and Computational Biology. The head of the "Laura Bassi Center of Optimized Structural Studies" explains: "We determined the structure of actinin using a technique called X-ray crystallography. It took us several years to produce sufficient high-quality protein necessary to obtain quality crystals for our analysis." With patience and innovative ideas the team eventually teased out the detailed structure of actinin, and in collaboration with Mathias Gautel at the British Heart Foundation Center of Research Excellence at KCL could also show how the protein is regulated.

The Structure is Similar to Fusilli Pasta

The structure showed that actinin forms a symmetrical complex made of two molecules. Each molecule has a head, short neck region and a rod-shaped body that looks like four fusilli pasta aligned in a zigzag.

The head of actinin binds actin whilst two small L-shaped parts sit at the end of the rod and interact with the neck of the other molecule. However, the structure revealed to be more than the sum of its parts: placing two diametrically opposing actinin molecules does not only allow simultaneous binding of actin and titin filaments and so anchoring them in the Z-disc, but also confers regulation.

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The Fatty Acid PIP2 Regulates actinin Function

"It was a long standing hypothesis, that the interaction of actinin with titin is regulated via a fatty acid molecule termed PIP2 which switches the molecule on and off. Our structural data now for the first time reveals how a fatty acid opens and closes the muscle protein actinin, modifying its ability to bind actin and titin", explains Kristina Djinović-Carugo.

If there is no PIP2, one of the L-shaped parts of an actinin molecule binds a titin-lookalike region in the neck of the opposing molecule. If PIP2 is present, the L-shaped part detaches from the neck and binds titin. The team complemented the structural picture of the interaction of PIP2 with actinin by analyzing the dynamic life of actinin in collaboration with Katharina Pirker (University of Natural Resources and Life Sciences Vienna), and Bojan agrovic (MFPL). Mathias Gautel's team also discovered that, when they destroyed actinin's binding site for PIP2 or locked it in a position which can permanently bind titin, ordered sarcomeres were lost; the muscle was broken.

Long-term Funding and International Collaborations were Essential

"Our findings give new insights into how muscle is built and flexes at the molecular level. This will help to better understand both inherited and acquired muscular diseases, and aid therapy development", sums up Kristina Djinović-Carugo. She adds:

"Without long-term funding through the FWF, the University of Vienna, the FFG, the EU-FP7 Marie Curie Action 'Networks for Initial Training' program, the British Heart Foundation, as well as the long standing collaborations with Mathias Gautel and Katharina Pirker, this research would have been impossible. This paper is the fruit of eight years of hard of labor!"

Publication in Cell

Euripedes de Almeida Ribeiro, Nikos Pinotsis, Andrea Ghisleni, Anita Salmazo, Petr V. Konarev, Julius Kostan, Bjoern Sjoeblom, Claudia Schreiner, Anton A. Polyansky, Eirini A. Gkougkoulia, Mark R. Holt, Finn L. Aachmann, Bojan agrovic, Enrica Bordignon, Katharina F. Pirker, Dmitri I. Svergun, Mathias Gautel and Kristina Djinović-Carugo: The structure and regulation of human muscle actinin. Cell. December 2014. DOI: dx.doi.org/10.1016/j.cell.2014.10.056

Attribution/Source(s):

This quality-reviewed article relating to our Bones and Joints section was selected for publishing by the editors of Disabled World due to its likely interest to our disability community readers. Though the content may have been edited for style, clarity, or length, the article "How Muscles Work Study Show Insight into Muscle Disorders" was originally written by University of Vienna, and published by Disabled-World.com on 2014-11-28 (Updated: 2021-03-15). Should you require further information or clarification, University of Vienna can be contacted at univie.ac.at. Disabled World makes no warranties or representations in connection therewith.

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