Skip to main content
Accessibility|Contact|Privacy|Terms|Cookies

Hereditary Spastic Paraplegia - Study Provides New Clues

  • Published: 2011-07-15 : Author: Rice University
  • Synopsis: First detailed account of biochemical workings of atlastin a protein produced by one of the genes linked to Hereditary Spastic Paraplegia.

Main Document

New research from Rice University and Italy's Eugenio Medea Scientific Institute is yielding clues about hereditary spastic paraplegia (HSP), a group of inherited neurological disorders that affect about 20,000 people in the United States.

A study in the July 5 issue of the Proceedings of the National Academy of Sciences offers the first detailed account of the biochemical workings of atlastin, a protein produced by one of the genes linked to HSP.

The primary symptoms of hereditary spastic paraplegia are progressive spasticity and weakness of the leg and hip muscles. This results from the slow degeneration of the nerves that carry signals from the spinal column to the legs, feet and toes. While scientists know that HSP can result from more than two dozen different genetic mutations, they don't know how the mutations cause nerve degeneration.

"We discovered a couple of years ago that atlastin plays a key role in building and maintaining an important internal compartment of healthy cells called the endoplasmic reticulum," said Rice biochemist James McNew, lead co-author of the new study. "HSP is known to primarily affect long nerve cells that can stretch from the lower back all the way to the base of the leg. It appears that atlastin plays a particularly crucial role in maintaining the health of these cells, and we want to know why."

McNew, associate professor of biochemistry and cell biology at Rice, and Andrea Daga, a scientist at the Medea Institute, determined in 2009 that atlastin was one of the rare breed of proteins that can cause membrane fusion.

"Membrane fusion is a fundamental process involved in many cell functions, but only a few proteins can initiate it," McNew said. "Until 2009, we thought all membrane fusion proteins operated in the same basic way. Atlastin was completely different because it's an enzyme that utilizes chemical energy to drive fusion. We really had to start from scratch to determine how it worked."

Membranes are both the brick walls and the zip-lock bags of microbiology. Every living cell is encased in a water-tight membrane of fatty acids, and all of the cell's interactions with the outside world are mediated by what can and cannot pass through that membrane. When a virus invades a healthy cell, it uses membrane fusion. When a sperm fertilizes an egg, it uses membrane fusion. Membranes are also used inside the cell, where they serve as biochemical reaction chambers, as cargo containers and as pipelines.

To find out how atlastin initiates membrane fusion, McNew, Daga and colleagues conducted experiments involving the fruit-fly version of atlastin, which is remarkably similar to the version found in humans. By selectively disabling portions of the protein and examining how those modifications affected atlastin's ability to promote membrane fusion in the test tube and cell cultures, the team gradually built a picture of how atlastin works. They found that pairs of atlastin proteins, each of which is anchored in a separate membrane, can bind to one another when both partners are functional enzymes. That action draws the separated membranes together and allows fusion to occur.

Additionally, they found that a small piece near the end of atlastin was required for fusion activity.

"Atlastin has a fairly short tail, which protrudes from the site where the protein is anchored into the membrane," Daga said. "When we deleted this tail, we found that the protein could not stimulate membrane fusion, even when the two copies bound together normally."

McNew said prior gene sequencing studies have show that some HSP patients have genetic mutations that result in defects in this critical tail region of the atlastin protein.

"This study, as well as work by others, suggests the area of the cell where atlastin promotes membrane fusion, the endoplasmic reticulum, is a good target for future studies into the causes of HSP," said McNew, a faculty investigator at Rice's BioScience Research Collaborative.

Study co-authors include Tyler Moss and Avani Verma, both of Rice; and Camilla Andreazza of the Medea Institute. The research was supported by the National Institutes of Health, the Mathers Charitable Foundation, the Italian Ministry of Health, the French Association Against Myopathies and Telethon Italy.

Discussion

• Discuss this article on our FaceBook Page.

Similar Topics

1 : Disability in South American Countries : Disabled World.
2 : Pride in Disability and Self-identifying as Disabled Aids Overall Well-being : Oregon State University.
3 : New Model to Support Workers with Disabilities : University of Cincinnati.
4 : Common Risk Factors That Impact Disability Rates : University of Michigan.
5 : Access Board Issues Guidance on the International Symbol of Accessibility : United States Access Board.
From our Disability Information section - Full List (65 Items)


Submit disability news, coming events, as well as assistive technology product news and reviews.


Loan Information for low income singles, families, seniors and disabled. Includes home, vehicle and personal loans.


Famous People with Disabilities - Well known people with disabilities and conditions who contributed to society.


List of awareness ribbon colors and their meaning. Also see our calendar of awareness dates.


Blood Pressure Chart - What should your blood pressure be, and information on blood group types/compatibility.





1 : Help Your Child in School by Adding Language to The Math
2 : 50% of Retirees Saw Little or No COLA Increase in Net 2018 Social Security Benefits
3 : Turnstone Endeavor Games Concludes with National Records Broken
4 : Spinning in Circles and Learning From Myself by Tsara Shelton
5 : St. Louis HELP Medical Equipment Donation Drive Generates Record-Breaking Results
6 : People Who Snore Suffer from Palate Nerve and Muscle Damage
7 : How Our Ancestors with Autistic Traits Led a Revolution in Ice Age Art
8 : Housing and Disabled People: Britains Hidden Crisis


Disclaimer: This site does not employ and is not overseen by medical professionals. Content on Disabled World is not intended to be a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of a physician or other qualified health provider with any questions you may have regarding a medical condition. See our Terms of Service for more information.

Reporting Errors: Disabled World is an independent website, your assistance in reporting outdated or inaccurate information is appreciated. If you find an error please let us know.

© 2004 - 2018 Disabled World™