Eating at Night, Sleeping By Day Alters Key Blood Proteins
Published : 2018-05-25 - Updated : 2019-12-29
Author : University of Colorado at Boulder - Contact: colorado.edu
Synopsis* : Research shows staying awake all night and sleeping all day for just a few days can disrupt levels and time of day patterns of more than 100 proteins in the blood. The study is the first to examine how protein levels in human blood, also known as the plasma proteome, vary over a 24-hour period and how altered sleep and meal timing affects them. By studying the actual proteins in the blood, researchers can study a broader array and get a better picture of what's happening in real time.
Staying awake all night and sleeping all day for just a few days can disrupt levels and time of day patterns of more than 100 proteins in the blood, including those that influence blood sugar, energy metabolism, and immune function, according to new University of Colorado Boulder research published in the journal PNAS this week.
"This tells us that when we experience things like jet lag or a couple of nights of shift work, we very rapidly alter our normal physiology in a way that if sustained can be detrimental to our health," said senior author Kenneth Wright, director of the Sleep and Chronobiology Laboratory and Professor in the Department of Integrative Physiology.
The study is the first to examine how protein levels in human blood, also known as the plasma proteome, vary over a 24-hour period and how altered sleep and meal timing affects them.
It also pinpointed 30 distinct proteins that, regardless of sleep and meal timing, vary depending upon what internal circadian time it is.
The findings could open the door for developing new treatments for night shift workers, who make up about 20 percent of the global workforce and are at higher risk for diabetes and cancer. It could also enable doctors to precisely time administration of drugs, vaccines and diagnostic tests around the circadian clock.
Woman sleeping on bed
"If we know the proteins that the clock regulates, we can adjust timing of treatments to be in line with those proteins," said lead author Christopher Depner, a postdoctoral researcher in the Department of Integrative Physiology.
The researchers recruited six healthy male subjects in their 20s to spend six days at CU's clinical translational research center, where their meals, sleep, activity and light exposure were tightly controlled.
On days one and two, the men stuck to a normal schedule. Then they were gradually transitioned to a simulated night-shift work pattern, in which they had eight hour sleep opportunities during the day and stayed up all night, eating then.
Researchers drew blood every four hours and used technology recently developed by Boulder-based SomaLogic, Inc to assess levels and time-of-day-patterns of 1,129 proteins. They found 129 proteins whose patterns were thrown off by the simulated night shift.
"By the second day of the misalignment we were already starting to see proteins that normally peak during the day peaking at night and vice versa," Depner said.
One of those proteins was glucagon, which prompts the liver to push more sugar into the bloodstream. When subjects stayed awake at night, levels not only surged at night instead of day but also peaked at higher levels. Long-term, this pattern could help explain why night-shift workers tend to have higher diabetes rates, Depner said.
The simulated night shift schedule also decreased levels of fibroblast growth factor 19, which has been shown in animal models to boost calorie-burning or energy expenditure. This fell in line with the finding that subjects burned 10 percent fewer calories per minute when their schedule was misaligned.
Thirty proteins showed a clear 24-hour-cycle, with the majority peaking between 2 p.m. and 9 p.m. The takeaway: When it comes to diagnostic blood tests - which are relied upon more often in the age of precision medicine - "timing matters," said Wright.
Previous studies have looked at time-of-day expression patterns of protein-coding genes in specific organs. By studying the actual proteins in the blood, researchers can study a broader array and get a better picture of what's happening in real time, Depner said.
He and Wright note that they kept all the study subjects in dim light conditions, so that light-exposure (which can also strongly affectthe circadian system) didn't influence results. Even without the glow of electronics at night, changes in protein patterns were rapid and widespread.
"This shows that the problem is not just light at night," Wright said. "When people eat at the wrong time or are awake at the wrong time that can have consequences too."
Eating at Night, Sleeping By Day Alters Key Blood Proteins | University of Colorado at Boulder (colorado.edu). Disabled World makes no warranties or representations in connection therewith. Content may have been edited for style, clarity or length.
You're reading Disabled World. Be sure to check out our homepage for further informative disability news, reviews, disability sports events, exclusive stories and how-tos. You can also find us on Twitter, Facebook, and LinkedIn.
Related Cardiovascular Documents
- 1: Irregular Heartbeat, Arrhythmia and Atrial Fibrillation : Uncovering genetic factors that contribute to underlying functional disturbance that results in atrial fibrillation.
- 2: Heart Disease Symptoms in Women : Heart disease a leading cause of death for women yet they are more likely than men to dismiss pain and delay seeking medical help.
- 3: 150 Systolic Blood Pressure in Seniors is OK : New guidelines suggest for adults over 60 keeping systolic blood pressure at 150 or less is adequate.
- 4: Need for New Approaches to High Blood Pressure Treatment : Wake Forest Baptist Medical Center research shows need for new approaches to treatment of hypertension or high blood pressure.
- 5: Aortic Aneurysms: Types, Causes, and Treatment : Information and general overview regarding Aortic Aneurysms that includes types, diagnoses, causes, symptoms and treatment methods.
*Disclaimer: Disabled World provides general information only. Materials presented are in no way meant to be a substitute for professional medical care by a qualified practitioner, nor should they be construed as such. Any 3rd party offering or advertising on disabled-world.com does not constitute endorsement by Disabled World. View our Advertising Policy for further information. Please report outdated or inaccurate information to us.
Journal: Disabled World. Language: English (U.S.). Author: University of Colorado at Boulder. Electronic Publication Date: 2018-05-25 - Revised: 2019-12-29. Title: Eating at Night, Sleeping By Day Alters Key Blood Proteins, Source: <a href=https://www.disabled-world.com/health/cardiovascular/plasma-proteome.php>Eating at Night, Sleeping By Day Alters Key Blood Proteins</a>. Retrieved 2021-04-18, from https://www.disabled-world.com/health/cardiovascular/plasma-proteome.php - Reference: DW#188-13446.