Tattoo Ink Nanoparticles Migrate to Lymph Nodes

Author: European Synchrotron Radiation Facility
Published: 2017/09/13 - Updated: 2026/01/10
Publication Details: Peer-Reviewed, Findings
Category Topic: Nanomedicine - Related Publications

Page Content: Synopsis - Introduction - Main - Insights, Updates

Synopsis: This research presents findings from a peer-reviewed study published in Scientific Reports by scientists at the European Synchrotron Radiation Facility and German research institutions. Using advanced X-ray fluorescence measurements and synchrotron technology, researchers documented how tattoo ink components - including titanium dioxide, organic pigments, and contaminants like nickel, chromium, and cobalt - migrate from injection sites to lymph nodes in nanoparticle form. The study marks the first time analytical evidence has confirmed this transport mechanism in human tissue samples, revealing that larger microparticles remain in the skin while only smaller nanoparticles reach the lymphatic system.

The findings matter because nanoparticles behave differently than larger particles, and their long-term deposition in lymph nodes may cause chronic enlargement and sustained exposure to potentially toxic elements. For people with tattoos who experience delayed healing, skin elevation, or persistent inflammation - conditions that can particularly affect individuals with compromised immune systems, including some people with disabilities and older adults - this research provides scientific grounding for understanding these adverse reactions. The work also identifies concerning gaps in tattoo safety regulations, since parlors check needle sterility but not ink chemical composition - Disabled World (DW).

Introduction

The elements that make up the ink in tattoos travel inside the body in micro and nanoparticle forms and reach the lymph nodes according to a study published in Scientific Reports on 12 September by scientists from Germany and the ESRF, the European Synchrotron, Grenoble (France). It is the first time that there is analytical evidence of the transport of various organic and inorganic pigments and toxic element impurities as well as in depth characterization of the pigments ex vivo in tattooed tissues. Two ESRF beamlines have been crucial in this breakthrough.

Main Content

The reality is that little is known about the potential impurities in the colour mixture applied to the skin. Most tattoo inks contain organic pigments, but also include preservatives and contaminants like nickel, chromium, manganese or cobalt.

"When someone wants to get a tattoo, they are often very careful in choosing a parlour where they use sterile needles that haven't been used previously. No one checks the chemical composition of the colours, but our study shows that maybe they should", explains Hiram Castillo, one of the authors of the study and scientist at the ESRF.

TiO2

Besides carbon black, the second most common ingredient used in tattoo inks is titanium dioxide (TiO2), a white pigment usually applied to create certain shades when mixed with colorants. TiO2 is also commonly used in food additives, sun screens, paints. Delayed healing, along with skin elevation and itching, are often associated with white tattoos, and by consequence with the use of TiO2.

Scientists from the ESRF, the German Federal Institute for Risk Assessment, Ludwig-Maximilians University, and the Physikalisch-Technische Bundesanstalt have managed to get a very clear picture on the location of titanium dioxide once they get in the tissue. This work was done on the ESRF beamlines or experimental stations, ID21 and ID16B.

This AI image shows a close-up view of a person's inner forearm featuring a large, colorful tattoo that runs vertically along the arm. At the center of the design is a highly detailed tiger's head with its mouth open in a roar, showing sharp teeth and fangs, and intense amber-colored eyes that face forward. The tiger's fur is rendered in realistic shades of orange, black, and white, with fine linework that gives it a textured, lifelike appearance. Surrounding the tiger are several large red flowers resembling peonies, with layered petals and dark gray leaves that frame the animal above and below, creating a balanced, symmetrical composition. The skin tone of the arm is light, and the background is softly blurred and dark, which makes the tattoo's bold colors and intricate details stand out clearly.

The hazards that potentially derive from tattoos were as yet only investigated by chemical analysis of the inks and their degradation products in vitro.

"We already knew that pigments from tattoos would travel to the lymph nodes because of visual evidence: the lymph nodes become tinted with the colour of the tattoo. It is the response of the body to clean the site of entrance of the tattoo. What we didn't know is that they do it in a nano form, which implies that they may not have the same behaviour as the particles at a micro level. And that is the problem: we don't know how nanoparticles react", explains Bernhard Hesse, one of the two first authors of the study and ESRF visiting scientist.

X-ray fluorescence measurements on ID21 allowed the team to locate titanium dioxide at the micro and nano range in the skin and the lymphatic environment. They found a broad range of particles with up to several micrometres in size in human skin but only smaller (nano) particles transported to the lymph nodes. This may lead to the chronic enlargement of the lymph node and lifelong exposure. Scientists also used the technique of Fourier transform infrared spectroscopy to assess biomolecular changes in the tissues in the proximity of the tattoo particles.

Altogether the scientists report strong evidence for both migration and long-term deposition of toxic elements and tattoo pigments as well as for conformational alterations of biomolecules that are sometimes linked to cutaneous inflammation and other adversities upon tattooing.

Then next step for the team is to inspect further samples of patients with adverse effects in their tattoos in order to find links with chemical and structural properties of the pigments used to create these tattoos.

Insights, Analysis, and Developments

Editorial Note: What makes this research particularly significant is its methodology: the team didn't rely on chemical analysis of inks in test tubes but actually tracked pigment migration in human tissue using some of Europe's most sophisticated imaging technology. The revelation that our bodies respond to tattoos by attempting to "clean" the injection site, only to relocate problematic nanoparticles to lymph nodes where they accumulate indefinitely, raises questions that extend beyond aesthetics into public health policy. As the researchers prepare to examine tissues from patients with adverse tattoo reactions, their work may finally provide the evidence base needed to establish safety standards for an industry that has operated largely without chemical oversight - a gap that affects millions of tattooed individuals who never questioned what they were permanently placing under their skin - Disabled World (DW).

Attribution/Source(s): This peer reviewed publication was selected for publishing by the editors of Disabled World (DW) due to its relevance to the disability community. Originally authored by European Synchrotron Radiation Facility and published on 2017/09/13, this content may have been edited for style, clarity, or brevity.