Making Stem Cells Behave Like Human Embryos

Introduction

Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells

A serendipitous discovery in the lab has the potential to revolutionize embryo models and targeted drug therapies. Materials scientists at UNSW Sydney have shown that human pluripotent stem cells in a lab can initiate a process resembling the gastrulation phase - where cells begin differentiating into new cell types - much earlier than occurs in mother nature.

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For an embryo developing in the womb, gastrulation occurs at day 14. But in a lab at UNSW's Kensington campus, Scientia Associate Professor Kris Kilian oversaw an experiment where a gastrulation-like event was triggered within two days of culturing human stem cells in a unique biomaterial, as it turned out, set the conditions to mimic this stage of embryo development.

"Gastrulation is the key step that leads to the human body plan," says Associate Professor Kilian.

"It is the start of the process where a simple sheet of cells transforms to make up all the tissues of the body - nerves, cardiovascular and blood tissue, and structural tissue like muscle and bone. But we haven't been able to study the process in humans because you can't study this in the lab without taking developing embryonic tissue."

"So it's fascinating that we were able to see this happening in vitro."

The achievement, which was reported today in the journal Advanced Science, has not only implications for our understanding of human embryonic development but also new treatments in medicine, including cell therapy, targeted drug development, and CRISPR gene-editing technologies.

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The Most Important Time in Your Life

Developmental biologist Lewis Wolpert once said:

"It is not birth, marriage, or death, but gastrulation, which is truly the most important time in your life."

Gastrulation is the key event in an embryo's development when a mass of undifferentiated cells begins the first steps of a long journey in the womb toward the formation of a human being. This is one of the reasons that work on embryos left over from IVF is forbidden beyond 14 days when gastrulation occurs.

Associate Professor Kilian says that up until now, it has been difficult to study this process in humans because of obvious ethical constraints.

"Controlling gastrulation using materials alone will provide an entirely new way for studying human development," he says. "We currently can't do this because embryo research beyond 14 days is often viewed as unethical, and it's currently impossible in vivo because you'd need to observe an embryo in a pregnant human mother."

But while there are animal models to study - such as mice and zebrafish - and other researchers have induced gastrulation-like events in the lab using chemicals including growth factors, this is the first time culture conditions alone have initiated gastrulation outside of a human body.

"Our method could lead to a new approach to mimic human embryogenesis outside of a person," Associate Professor Kilian says.

Miniature Organs and Crispr Gene Splicing

In the medical sciences, the ability to induce gastrulation in 'synthetic' embryos like those created by the UNSW team could also help create body tissue or even miniature organs based on a patient's genetic code. These so-called 'organoids,' which are barely visible to the naked eye, are already being developed using stem cells for medical research, such as testing the effectiveness of certain drugs. But the process requires chemicals to stimulate the cells into forming differentiated organ tissue which is time-consuming and expensive.

Associate Professor Kilian says controlling gastrulation using only hydrogel materials to stimulate what happens naturally could be a quicker and more cost-effective solution.

"The thing that excites us about this is the potential to make therapeutically useful cells much faster and more reproducible," says Associate Professor Kilian.

"Our method could provide a way to initiate 'organogenesis' - with an array of hundreds of well-defined cell aggregates in a single well - leading to faster and more well-defined structures that could then be turned into brain, liver, gut, potentially any solid organ tissue."

"This approach could also revolutionize drug development, including RNA and CRISPR/Cas9 approaches, by providing a more reproducible way to mimic human tissue in a lab. For instance, you could make an organoid from a patient's cells, then test therapies to correct mutations or restore function."

A Hydrogel Home is Just Right

The secret to the success of the UNSW team's work in the lab is in the structure of the culture that the stem cells were seeded into. Using a technique adapted from the semiconductor industry, defined regions are fabricated across a hydrogel for cells to stick to. This combination of geometric confinement and the soft gel that mimics the surface of a human uterus coaxes the cells to start gastrulation-like processes.

"We discovered that if you take pluripotent stem cells and put them in a very confined and soft environment, it's akin to what the cells might experience in a mother's uterus," says Associate Professor Kilian.

"That viscoelastic, soft, squishy material gives them just enough cues to initiate this gastrulation-like process all on their own."

This contrasts greatly with the standard practice used in labs more recently that forces a type of gastrulation process using growth factors and chemical supplements on hard plastic or glass dishes.

"Unsurprisingly, previous research culturing stem cells on glass or plastic has failed to recapitulate the signals that happen in a body. But using our soft substrates mimicking embryonic tissue, we can coax the cells to spatially organize and begin the early morphogenesis that could ultimately create a person."

But, Associate Professor Kilian cautions while the team has discovered the conditions that emulate the first stage of gastrulation, it doesn't appear to go any further.

"We can't make a person this way," he says.

"This method only demonstrates the early but very crucial stage in development. The impact lies in studying this all-important stage of human development and using the generated structures for developing therapies."

Serendipity Can Be a Big Part of Discovery

As with most great scientific discoveries, luck played a role. The team wasn't actively looking to bring on gastrulation when they dropped some stem cells onto the hydrogel substrate.

Lead author Dr. Pallavi Srivastava was surprised by what she observed.

"Initially, I was trying to get stem cells to attach to our hydrogels and planned to differentiate them conventionally," she says.

"The difference between cells cultured on glass and those on our gels was very striking. I remember thinking, 'wow, something is going on here. I need to investigate. This led to a big shift in my project and this exciting discovery."

The researchers are hopeful they can continue exploring the benefits of their discovery by understanding how materials can guide embryogenesis and beyond. Associate Professor Kilian says that while this finding is exciting, more work is needed to guide the gastrulation-like processes to form useful tissues.

"This is the first step in what we hope is a platform technology for producing useful tissue models. Triggering gastrulation is not enough - now we need to provide other signals to keep differentiation going."

Discovering the next set of materials signals may allow the creation of virtually any solid tissue for research purposes, Associate Professor Kilian says, and for generating useful cell types for regenerative medicine.

"Considering pluripotent stem cells can now be generated from blood or tissue samples, the future is wide open for regenerating tissues and organs from a patient's cells."

Attribution/Source(s):

This peer reviewed publication was selected for publishing by the editors of Disabled World due to its significant relevance to the disability community. Originally authored by University of New South Wales, and published on 2022/12/18 (Edit Update: 2023/01/03), the content may have been edited for style, clarity, or brevity. For further details or clarifications, University of New South Wales can be contacted at unsw.edu.au. NOTE: Disabled World does not provide any warranties or endorsements related to this article.

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Cite This Page (APA): University of New South Wales. (2022, December 18 - Last revised: 2023, January 3). Making Stem Cells Behave Like Human Embryos. Disabled World. Retrieved December 10, 2024 from www.disabled-world.com/news/research/stemcells/embryo-cells.php

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